Sanger Sequencing

Automated DNA sequencing is an indispensable tool for obtaining high-quality sequences that can be used for publication in scientific journals and to be included in databases.
Microgem Lab offers "Sanger Sequencing" service. Single strand DNA (ssDNAs) or double strand DNA (dsDNAs) and PCR products can be sequenced with reads greater than 800 bp and a reliability of > 99%.


Sample Requirements

Sample Requirements • Purified PCR products <1 kb, 5 ng/100 bp, 15 ul • Purified PCR products ≥1kb, 100 ng, 15 ul • Non purified PCR products 20 ul • Primers 10 ul at 2 uM • Plasmid 400 ng • DNA samples are required to have an OD 260/280 ratio ≥1.7-2.0 and 260/230 ratio≥1.8-2.2 Please supply a shot of agarose gel.


Standard Sequencing & Sequencing "Primer Walking"

Standard Sequencing (plasmids and PCR products). Sequencing "Primer Walking" (sequencing of DNA fragments in the range of 1 to 10 kb). On request, it is possible to purify PCR products or prepare plasmid DNA from bacterial transformations.


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